04/14/2004
Sopranos Singing Softly
Last week I took my first walk outdoors after returning home from Marco Island. As I walked by the home of a former mayor of our town, what should I see in his yard but two wild turkeys! This was the first time I’d seen turkeys wandering about in our town. The next day my wife saw a turkey in our own back yard. This unexpected wildlife in our vicinity makes life more interesting but I miss the sounds of the surf and the seabirds during my walks on the beach on Marco.
Let’s talk about some other sounds. I understand that a new trend is for expectant parents to acquire ultrasound portraits of their unborn children to display and/or preserve for posterity. Ultrasound has its more serious uses, of course, notably in other forms of medical diagnosis. It was ultrasound that detected the possibility of a tumor in my kidney that was confirmed by MRI. It was ultrasound that was used to help initiate the purported nuclear fusion in the “sonofusion” experiments we discussed a few weeks ago.
We aren’t biologically equipped to hear ultrasound but there are other interesting sounds that can be heard. One of them, if real, must be about the softest sound imaginable. I must caution you that, so far as I know, the research on this soft sound has not yet been published in a scientific journal and there is some skepticism about its reality. I learned of it in an article titled “Signal Discovery” by Mark Wheeler in the March issue of Smithsonian magazine. The article deals with the work of James Gimzewski, a chemist at UCLA. A word about Gimzewski’s credentials – he came to UCLA by way of IBM’s Zurich research lab where he was a world leader in nanotechnology. He and his coworkers in Zurich accomplished feats such as making the world’s smallest abacus with beads less than a nanometer in size or a spinning molecular propeller less than a couple nanometers in size. In other words, he knows how to handle really tiny stuff!
Jim was lured from IBM by UCLA and his work there has been influenced significantly by the events of 9/11/2001. He was at a conference in Italy where he met an Italian medical researcher, Carlo Ventura. Ventura was interested in childhood heart abnormalities and was working with stem cell precursors to heart cells. Gimzewski was taken with the fact that living heart cells keep beating when placed in a dish with appropriate nutrients. If something vibrates, it must produce a sound. After all, sound is produced by something vibrating, your radio speaker, for example, that pushes and pulls on the molecules in the air. The pushing and pulling generates the sound waves that we hear if the vibrations are in our frequency range.
Gimzewski wondered if he couldn’t “hear” the heart cells beating and Ventura said that he would send him some of his heart cells to study. Unfortunately, 9/11 intervened and by the time a package of stem cells from Sardinia hit U. S. Customs, biological warfare came to mind and the shipment was held up until it was clear the cells posed no threat. By the time they reached Gimzewski, they were beating no longer! Gimzewski wasn’t to be denied, however, and asked his biochemistry colleagues at UCLA if they couldn’t give him some yeast cells to listen to. LA Weekly.com, an alternative newspaper’s Web site has an interview of Gimzewski with Margaret Wertheim, who describes him as easily mistaken for a refugee from a British rock band. I imagine his biochemistry colleagues thought his idea of listening to yeast cells “singing” rather odd, to say the least.
In the past, we’ve talked about various kinds of so-called scanning probe microscopes that permit the imaging of individual atoms. The first of these, known as the scanning tunneling microscope, was invented at the IBM Zurich lab where Gimzewski did his nano work. At UCLA, he and his graduate student, Andrew Pelling, set to work using the atomic force microscope (AFM), another type of scanning probe microscope. The AFM has a tiny point that is normally dragged ever so lightly across a surface and a computer converts the bumps and valleys into a picture. Gimzewski and Pelling took an AFM and, instead of dragging the point, held it lightly on the surface of a yeast cell. Sure enough, they found that the cell membrane did “beat”, raising and lowering the point.
Surprisingly, the cells were vibrating quite rapidly, around 1,000 times a second. The AFM measurements indicated that the cell wall moved up and down by 3 nanometers, in the neighborhood of 10 atom diameters. The distance the cell wall moves in and out determines how loud the sound is, while the frequency determines the pitch. Actually, if our ears were supersensitive, we should be able to hear the yeast cells singing. On the musical scale, the note C that lies two octaves above middle C corresponds to a frequency of 1,056 cycles per second. When I plunked that key on our piano, my aging ears had no trouble hearing it. At about a thousand cycles per second, those vibrating yeast cells are “singing soprano”, as the LA Weekly article put it.
Of course, with only a movement of a few nanometers, the volume is too low for us to hear. It’s just as well. If cells do sound off, we would be inundated by sound if we could hear them all! Gimzewski and Pelling next decided to sprinkle the cells with alcohol. The cells started vibrating at a higher frequency, a higher pitch. Were they screaming before they died? When the cells were dead, they emitted a low (very low!) rumbling sound that Gimzewski attributes to random atomic motion, not to rhythmic beating.
The researchers have looked at other types of cells and found that they emit sound at different frequencies. For example, bone cells sing at a lower frequency than the yeast cells. Mutant yeast cells were found to sound different from normal yeast cells. This has spurred the hope that listening to cells might prove to be a good diagnostic tool for various diseases. As I mentioned, Gimzewski hadn’t published his results as of the time the article was written and there are skeptics who think the vibrations could have other origins, perhaps even in the tip of the AFM itself. Gimzewski and his colleagues agree that more work is needed to determine whether what he calls “sonocytology” will be of practical value.
I listened to an interview of Gimzewski by Quentin Cooper of the BBC on the BBC Web site. The interview took place last year and included another interviewee, Matthew Cooper, the chief scientific officer of a company called Abukio. I don’t think the two Coopers are related. Matthew and his colleagues are “listening” to viruses using a completely different technique. An article by Helen Pearson, dated August 31, 2001, on the Nature Web site indicates that they coat a quartz crystal about the size of a dime with an antibody to which human herpes virus was attached.
Quartz crystals in an electrical field vibrate. As the voltage on the crystal is increased the crystal vibrates faster and faster and finally the bonds between the antibodies and the viruses are broken. When a bond breaks, a burst of sound is emitted that is likened to a rifle shot. The characteristics of the sound are apparently dependent on the nature of the virus and the strength of the bond being broken. In these cases the frequency of the sound is beyond our hearing capability. Once again, the hope here is that the distinctive sounds emitted by different viruses or other biological entities may prove to be useful in the medical field.
For example, listening to cells or viruses might eliminate the need for some expensive and time-consuming tests. As I look forward to a CT scan next week, with the associated swallowing and injection of dyes, I certainly would prefer to have some cells “sing” for the diagnosticians. I wish Gimzewski and Cooper well in their quests.
Allen F. Bortrum
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